Isolation and Characterization of Alkaloids from Carica Papaya Linn

The carpaine was isolated from Carica papaya L. leaf, was identified by means of Fourier transform infrared spectroscopy, mass spectrometry, nuclear magnetic resonance, HPLC and was determined by LCMS, The powder of dried leaves was macerated and extracted with Methanol/water/HCl (89:10:1 V/V/V) to produce crude alkaloid. The extract was fractionated on a silica gel column chromatography using methanol/chloroform (5:95 v/v) as the eluate. The major fraction was isolated according to Rf values of thin-layer chromatography (TLC) (silica gel, methanol/chloroform [15:85 v/v]) exposed by Dragendroff's reagent. The extraction procedure, sample preparation, and LCMS conditions were evaluated and optimized. The study was done on pharmacological studies on leaves of Carica Papaya Linn.